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The calculation of FSR by using muscle intracellular enrichment as the precursor ( Figure 4A) or by using blood as the precursor ( Figure 4B) produced similar results. For the calculation of FSR by using muscle fractionation as the precursor, we did not control for the fact that only the first muscle fragment (F1) was obtained, which is the most typical stage of muscle cell differentiation. In both case, F3 and F4 were equally effective as predictors, which again demonstrates that muscle fractionation alone is sensitive to muscle-specific muscle fiber fractionation, as expected with the use of whole-body magnetic resonance tomography (MRT) for muscle tracing. Using two different estimation schemes (one of which uses muscle fractionation as the precursor) showed very close correlation between the two metrics (r = .74; R2 = .76). As for the calculation of FSR by using blood as the precursor, the calculation of FSR by using the whole-body estimation scheme remained similar.To further prove that the expression of FSR by the GIRK1/2-targeted receptor was dependent upon the muscle fiber type, we performed a kinetic analysis of the muscle contraction. As in the previous studies  , we used an average of four different parameters to determine the muscle fiber type from the GIRK1/2 receptor knockout mice (see Materials and Methods at the bottom of the page). Using five different parameters (Ki, T, AUC, Ki, and T/AUC) and assuming the muscle fiber types as described in the previous studies  , we could estimate the mean amplitude of the muscle contraction and derive a velocity of the muscle contraction over time. The calculated velocity calculated from four different parameters obtained a mean velocity of .85 m/s, and the calculated mean amplitude obtained the mean amplitude of the muscle contraction was .91, corresponding to a mean torque of .89 N/m when the target muscle fiber was the myotube.DiscussionThe present work presents a novel, and yet novel, interpretation of the current model of muscle differentiation. In contrast to previous studies reporting that muscle fiber types depend on the muscle fiber type [26–28], the current analysis provided the first evidence that muscle fiber types depend on the skeletal muscle fiber type. We also presented the first evidence that muscle fiber types are in turn related to the expression of the GIRK1/2-targeted receptor. This conclusion is supported by these data. Moreover, our analyses demonstrated that each differentially expressed genetic construct within the muscle fiber type affects the rate and magnitude of the muscle contraction by influencing both the time of the muscle contraction andSimilar articles: